Transduction of Intracellular and Intercellular Dynamics in Yeast Glycolytic Oscillations
Glycolysis, oscillating system (9 variables)
Jana Wolf,* Jutta Passarge,†,‡ Oscar J.G. Somsen,† Jacky L. Snoep,† Reinhart Heinrich,* and Hans V. Westerhoff†
*Humboldt University, Institute of Biology, Theoretical Biophysics, Invalidenstrasse 42, D-10115 Berlin, Germany, †BioCentrum Amsterdam, Departments of Molecular Cell Physiology and Mathematical Biochemistry, BioCentrum Amsterdam, De Boelelaan 1087, NL-1081 HV Amsterdam, European Union
Under certain well-defined conditions, a population of yeast cells exhibits glycolytic oscillations that synchronize through intercellular acetaldehyde. This implies that the dynamic phenomenon of the oscillation propagates within and between cells. We here develop a method to establish by which route dynamics propagate through a biological reaction network. Application of the method to yeast demonstrates how the oscillations and the synchronization signal can be transduced. That transduction is not so much through the backbone of glycolysis, as via the Gibbs energy and redox coenzyme couples (ATP/ADP, and NADH/NAD), and via both intra- and intercellular acetaldehyde.
Biophysical Journal Volume 78 March 2000 1145–1153